Sequencing technology – PacBio – Pacific Biosciences

HiFi sequencing

HiFi sequencing is recognized as the most accurate and complete sequencing technology:

• PacBio HiFi offers 21× fewer errors vs Oxford Nanopore, >6× fewer errors vs Illumina in PrecisionFDA benchmarking of total errors (SNV + indel + SV)
• Read lengths up to 25 kb allow you to span large structural variants and challenging repetitive regions, and to sequence full-length transcripts
• High accuracy provides over 99.99% accurate sequencing results
• Uniform coverage enables sequencing through regions that are inaccessible to other technologies
• Epigenetics can be explored through direct detection of base modifications during sequencing

Learn more See how

Sequencing by binding

SBB demonstrates greater accuracy and consistency compared to current short-read sequencing-by-synthesis (SBS) technologies. An improved limit of detection, customer flexibility, and less computational burden have the potential to enable results in research and clinical markets.

• Reads achieve <0.1% “true human variation level” through 200 cycles
• Increased read accuracy results in 2x fewer false positive variant calls than SBS

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